fop flash vector Search Results


top/fop flash vector  (Promega)
90
Promega top/fop flash vector
miR-140-3p represses Wnt/β-catenin signaling in LUAD. mRNA expression levels of (A) total β-catenin, (B) c-Myc and (C) cyclin D1 in A549 and Calu3 cells following transfection with miR-140-3p or the control mimic. (D) Protein expression levels of total β-catenin, c-Myc and cyclin D1 following transfection with miR-140-3p or the control mimic. (E) <t>TOP/FOP</t> flash activity in A549 and Calu3 cells following transfection with miR-140-3p or the control mimic. Data are presented as the mean ± standard deviation from three independent experiments. * P<0.05 and ** P<0.01 vs. control mimic. miR, microRNA; LUAD, lung adenocarcinoma.
Top/Fop Flash Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fop+flash+vector/top+fop+flash+vector/pmc07388557-85-7-10
Average 90 stars, based on 1 article reviews
top/fop flash vector - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
β-catenin/tcf firefly luciferase reporter vectors fop-flash  (Promega)
90
Promega β-catenin/tcf firefly luciferase reporter vectors fop-flash
miR-140-3p represses Wnt/β-catenin signaling in LUAD. mRNA expression levels of (A) total β-catenin, (B) c-Myc and (C) cyclin D1 in A549 and Calu3 cells following transfection with miR-140-3p or the control mimic. (D) Protein expression levels of total β-catenin, c-Myc and cyclin D1 following transfection with miR-140-3p or the control mimic. (E) <t>TOP/FOP</t> flash activity in A549 and Calu3 cells following transfection with miR-140-3p or the control mimic. Data are presented as the mean ± standard deviation from three independent experiments. * P<0.05 and ** P<0.01 vs. control mimic. miR, microRNA; LUAD, lung adenocarcinoma.
β Catenin/Tcf Firefly Luciferase Reporter Vectors Fop Flash, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fop+flash+vector/%CE%B2+catenin+tcf+firefly+luciferase+reporter+vectors+fop+flash/pm32920660-65-1-18
Average 90 stars, based on 1 article reviews
β-catenin/tcf firefly luciferase reporter vectors fop-flash - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


miR-140-3p represses Wnt/β-catenin signaling in LUAD. mRNA expression levels of (A) total β-catenin, (B) c-Myc and (C) cyclin D1 in A549 and Calu3 cells following transfection with miR-140-3p or the control mimic. (D) Protein expression levels of total β-catenin, c-Myc and cyclin D1 following transfection with miR-140-3p or the control mimic. (E) TOP/FOP flash activity in A549 and Calu3 cells following transfection with miR-140-3p or the control mimic. Data are presented as the mean ± standard deviation from three independent experiments. * P<0.05 and ** P<0.01 vs. control mimic. miR, microRNA; LUAD, lung adenocarcinoma.

Journal: Experimental and Therapeutic Medicine

Article Title: miR-140-3p enhances cisplatin sensitivity and attenuates stem cell-like properties through repressing Wnt/β-catenin signaling in lung adenocarcinoma cells

doi: 10.3892/etm.2020.8847

Figure Lengend Snippet: miR-140-3p represses Wnt/β-catenin signaling in LUAD. mRNA expression levels of (A) total β-catenin, (B) c-Myc and (C) cyclin D1 in A549 and Calu3 cells following transfection with miR-140-3p or the control mimic. (D) Protein expression levels of total β-catenin, c-Myc and cyclin D1 following transfection with miR-140-3p or the control mimic. (E) TOP/FOP flash activity in A549 and Calu3 cells following transfection with miR-140-3p or the control mimic. Data are presented as the mean ± standard deviation from three independent experiments. * P<0.05 and ** P<0.01 vs. control mimic. miR, microRNA; LUAD, lung adenocarcinoma.

Article Snippet: Subsequently, cells were co-transfected with 100 ng TOP/FOP flash vector (Promega Corporation), internal control pRL-TK Renilla luciferase vector (10 ng) and control mimic or miR-140-3p mimics (50 nM) using Lipofectamine ® 2000.

Techniques: Expressing, Transfection, Activity Assay, Standard Deviation

miR-140-3p attenuates the stem cell-like properties in LUAD by suppressing Wnt/β-catenin signaling. (A) Protein and (B) mRNA expression levels of β-catenin in A549 and Calu3 cells following transfection with either the empty vector or vector expressing β-catenin. ** P<0.01 vs. vector. (C) Protein levels of total and nuclear β-catenin, * P<0.05 vs. vector + miR-140-3p mimic; ** P<0.01 vs. vector + control mimic. (D) TOP/FOP flash activity, * P<0.05 vs. vector + miR-140-3p mimic, ** P<0.01 vs. vector + control mimic. (E) number of spheres with diameter >100 µm formed by A549 and Calu3 cells. Scale bar, 100 µm. * P<0.05 vs. vector + miR-140-3p mimic, ** P<0.01 vs. vector + control mimic. (F) percentage of CD133 + cells analyzed using flow cytometry following co-transfection with either the empty vector or vector expressing β-catenin and miR-140-3p mimic or the control mimic. * P<0.05 vs. vector + miR-140-3p mimic, ** P<0.01 vs. vector + control mimic. Data are represented the mean ± standard deviation from three independent experiments. miR, microRNA; LUAD, lung adenocarcinoma; CD133, cluster of differentiation 133; ctnnb1 , β-catenin gene.

Journal: Experimental and Therapeutic Medicine

Article Title: miR-140-3p enhances cisplatin sensitivity and attenuates stem cell-like properties through repressing Wnt/β-catenin signaling in lung adenocarcinoma cells

doi: 10.3892/etm.2020.8847

Figure Lengend Snippet: miR-140-3p attenuates the stem cell-like properties in LUAD by suppressing Wnt/β-catenin signaling. (A) Protein and (B) mRNA expression levels of β-catenin in A549 and Calu3 cells following transfection with either the empty vector or vector expressing β-catenin. ** P<0.01 vs. vector. (C) Protein levels of total and nuclear β-catenin, * P<0.05 vs. vector + miR-140-3p mimic; ** P<0.01 vs. vector + control mimic. (D) TOP/FOP flash activity, * P<0.05 vs. vector + miR-140-3p mimic, ** P<0.01 vs. vector + control mimic. (E) number of spheres with diameter >100 µm formed by A549 and Calu3 cells. Scale bar, 100 µm. * P<0.05 vs. vector + miR-140-3p mimic, ** P<0.01 vs. vector + control mimic. (F) percentage of CD133 + cells analyzed using flow cytometry following co-transfection with either the empty vector or vector expressing β-catenin and miR-140-3p mimic or the control mimic. * P<0.05 vs. vector + miR-140-3p mimic, ** P<0.01 vs. vector + control mimic. Data are represented the mean ± standard deviation from three independent experiments. miR, microRNA; LUAD, lung adenocarcinoma; CD133, cluster of differentiation 133; ctnnb1 , β-catenin gene.

Article Snippet: Subsequently, cells were co-transfected with 100 ng TOP/FOP flash vector (Promega Corporation), internal control pRL-TK Renilla luciferase vector (10 ng) and control mimic or miR-140-3p mimics (50 nM) using Lipofectamine ® 2000.

Techniques: Expressing, Transfection, Plasmid Preparation, Activity Assay, Flow Cytometry, Cotransfection, Standard Deviation